| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
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| Autores: |
Santos,M.F.M.
Oliveira,F.C.A.C.
Kishimoto,R.K.
Borri,D.
Santos,F.P.S.
Campregher,P.V.
Silveira,P.A.A.
Hamerschlak,N.
Mangueira,C.L.P.
Duarte,F.B.
Crepaldi,A.H.
Salvino,M.A.
Velloso,E.D.R.P.
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| Data: |
2019-01-01
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| Ano: |
2019
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| Palavras-chave: |
Cytogenetics
Karyotype
Leukemia
Myelodysplasia
Myeloproliferative disease
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| Resumo: |
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/1414-431x20188194
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.52 n.2 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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